Phytoconstituent Isolation and Cytotoxicity Evaluation of the Egyptian Cassia occidentalis L. Possessing Selective Activity against Lung Carcinoma

Ethyl acetate fraction column chromatographic analysis was used to isolate eleven compounds (numerically tagged 1–10) from Cassia occidentalis L. in this study. Two unique metabolites, including a neolignan compound designated as occidentalignan I (9) and flavonoidal glycoside, chrysin-7-O-α-L-rhamnopyranosyl (10), were identified, while silybin A (8) the first flavonolignan be isolated Fabaceae family. Four compounds, β-sitosterol-3-O-β-D-glucopyranoside (1), stigmasterol-3-O-β-D-glucopyranoside (2), betulinic acid (3), vanillic (4) C. for time. In addition, four known cinnamic (5), p-hydroxybenzoic (6), β- resorcylic (7), citric (11), also detected. The in-vitro cytotoxicity assessment of methanolic extract on seven cancer cell lines, A-549, Colo-205, Huh-7, HCT-116, PANC-1, SKOV-3, BNL, demonstrated its selective potent lung cells without affecting normal BNL cells. contrast, showed moderate activity Colo-205 Huh-7 nearly no SKOV-3 lines. These results suggest that is an excellent candidate with potential antiproliferative against cancer; however, further studies are necessary clarify mechanism action.